Detection of Cutaneous Leishmaniasis Based on ITS1 Gene by PCR-RFLP Technique
نویسندگان
چکیده
منابع مشابه
Cutaneous leishmaniasis in French Guiana: revising epidemiology with PCR-RFLP
BACKGROUND The development of polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique for species identification among patients presenting leishmaniasis allowed to better determine the main circulating species in French Guiana. METHODS A descriptive study of the Leishmania species was identified, and their spatiotemporal distribution was conducted using patient...
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Three species of L. tropica, L. major and L. infantum are known as main causal agents of leishmaniasis have been reported in Iran. Since cutaneous leishmaniasis (CL) is endemic in North East of Iran, in the present work, 50 Leishmania positive isolates from human cases in Mashhad (Center of Razavi province, North East of Iran), were genotyped by means of polymerase chain reaction-restriction f...
متن کاملDetection of filarial parasites in domestic cats by PCR-RFLP of ITS1.
Lymphatic filariasis has been targeted by the World Health Organization (WHO) to be eliminated by the year 2020. In addition to chemotherapy and vector control, the control of reservoir hosts is necessary for the control program to succeed. Malayan filariasis, caused by Brugia malayi, is endemic in the South of Thailand where domestic cats serve as the major reservoir host. However, in nature, ...
متن کاملDetection of Cutaneous Leishmaniasis by PCR in Fasa district in 2012
Background & Objective: Leishmaniasis is a common disease between humans and animals caused by protozoa of the genus Leishmania spp. It is transmitted by the female species of Phlebotomus.The most common form of leishmaniasis is the cutaneous form seen as either dry (urban) or wet (Rural). Detection of cutaneous leishmaniasis species is not reliable by microscopy. Among the methods, PCR is usef...
متن کاملDetection of Drug Resistance Gene in Cutaneous Leishmaniasis by PCR in Some Endemic Areas of Iran
BACKGROUND Cutaneous leishmaniasis is still a health problem in many rural and urban regions of Iran and drug resistance has emerged as a major impediment in the treatment of leishmaniasis. This study aims to determine the drug resistance gene in cutaneous leishmaniasis by PCR in some endemic areas of Iran. METHODS Ninety seven samples were collected from ulcers of leishmaniasis patients from...
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ژورنال
عنوان ژورنال: Egyptian Academic Journal of Biological Sciences, E. Medical Entomology & Parasitology
سال: 2018
ISSN: 2090-0856
DOI: 10.21608/eajbse.2018.22719